Which creatinine measurement method uses the alkaline picrate reaction?

Which creatinine measurement method uses the alkaline picrate reaction?

• A. Diacetyl monoxime BUN method
• B. Hexokinase glucose method
• C. Alkaline picrate creatinine method
• D. Biuret total protein method

Answer: (C)

The test is about recognizing the creatinine measurement method that uses the alkaline picrate reaction. In this method, creatinine reacts with picrate (picric acid) in an alkaline medium to form a colored complex, the intensity of which is measured spectrophotometrically and correlates with the creatinine concentration. This approach is known as the alkaline picrate creatinine method or the Jaffe reaction. It’s distinct from the diacetyl monoxime BUN method, which measures urea; the hexokinase method, which is used for glucose; and the biuret method, which measures total protein. The alkaline picrate method is historically common but can be influenced by other substances that react with picrate, hence some versions include corrections to improve specificity.

The test is about recognizing the creatinine measurement method that uses the alkaline picrate reaction. In this method, creatinine reacts with picrate (picric acid) in an alkaline medium to form a colored complex, the intensity of which is measured spectrophotometrically and correlates with the creatinine concentration. This approach is known as the alkaline picrate creatinine method or the Jaffe reaction. It’s distinct from the diacetyl monoxime BUN method, which measures urea; the hexokinase method, which is used for glucose; and the biuret method, which measures total protein. The alkaline picrate method is historically common but can be influenced by other substances that react with picrate, hence some versions include corrections to improve specificity.