RFLP analysis detects genetic variation based on differences in which factor?

RFLP analysis detects genetic variation based on differences in which factor?

• A. Restriction enzyme cutting sites
• B. Nucleotide composition of proteins
• C. RNA splicing patterns
• D. Post-translational modifications

Answer: (A)

RFLP analysis hinges on DNA sequence differences that alter where restriction enzymes cut. Each enzyme recognizes a specific short DNA motif, so a polymorphism—like a SNP or small insertion/deletion—can create or abolish a cutting site. After digestion and gel separation, individuals show different fragment lengths that reveal genetic variation. This method focuses on DNA-level changes, not on protein amino acid composition, RNA splicing patterns, or post-translational modifications, which are outside what RFLP detects.

RFLP analysis hinges on DNA sequence differences that alter where restriction enzymes cut. Each enzyme recognizes a specific short DNA motif, so a polymorphism—like a SNP or small insertion/deletion—can create or abolish a cutting site. After digestion and gel separation, individuals show different fragment lengths that reveal genetic variation. This method focuses on DNA-level changes, not on protein amino acid composition, RNA splicing patterns, or post-translational modifications, which are outside what RFLP detects.